![]() When asked whether the Democrats could keep the House, Biden said there was "a possibility," but acknowledged it would be close. Several key Senate races, including those in Nevada and Arizona, remain too close to call. Though Democrats are expected to lose control of the House of Representatives, control of the Senate is still up in the air. Raphael Warnock and Herschel Walker are going to a runoff in Georgia The Slotkin-Barrett race was among the most expensive House contests nationwide, with more than $27 million in spending by the campaigns and outside groups.Elections Sen. Liz Cheney, a conservative Republican who lost her own reelection bid in Wyoming's GOP primary after breaking with Trump over the Capitol attack. ![]() Slotkin drew a high-profile endorsement from U.S. Barrett said he was "pro-life" but that the issue was for states, not the federal government, to decide. Slotkin, an abortion rights supporter, described Barrett as rigidly opposed, with no exceptions for rape victims. ![]() Slotkin, a former CIA Middle East analyst, ran on a record that included support for her party's flagship legislation aimed at reducing carbon emissions, slashing drug costs and taxing large companies.īarrett said during a debate he would have opposed the bill as too expensive and inflationary. She defeated Republican Tom Barrett, a state senator and Army veteran. Elissa Slotkin narrowly won a third term in the 7th District, which includes Lansing, the state capital. He has also stressed his willingness to work across party lines, and promised to make improvements in public safety and education. In a region heavily dependent on agriculture, he’s been critical of state water management and puts water and agriculture at the top of his issues list. His opponent, Democrat Adam Gray, is a state legislator known as a moderate. The top finisher in the June primary was Republican John Duarte, a businessman and major grape and almond farmer whose top priorities include obtaining adequate water supplies for farmers in the drought-wracked state - a perennial issue in the Central Valley - along with addressing inflation and crime. That provides a possible opening for the GOP, despite the 14-point Democratic registration advantage. But the most likely voters tend to be white, older, more affluent homeowners, while working-class voters, including many Latinos, are less consistent in getting to the polls. Like other districts in California’s farm-belt region, the 13th District has a prominent Democratic tilt and a large Latino population. Hodge was seen as a rising star in Democratic politics but was hampered by the slight Republican registration advantage in the wealthy 1st Congressional District that covers parts of northeast Phoenix, Scottsdale, Paradise Valley and Fountain Hills. Schweikert survived a second straight election where he was dogged by ethics issues, following violations of campaign finance rules and laws barring the use of congressional staff for campaign work. David Schweikert defeated Democrat Jevin Hodge to gain a seventh term representing the northeast Phoenix suburbs’ 1st District. ![]() Tap or click for a detailed race breakdown:ĪZ-1: David Schweikert (R) wins reelection Here’s a look at how some of the key races are turning out.
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![]() Marine organisms have been less explored than mammals and microorganisms even though there are higher probabilities to find enzymes that have different specifics towards non-natural substrates in those environments. The sea anemone Condylactis gigantean is a representative of this family. Recent studies about marine coelenterates venom have demonstrated affinity to that from snakes, based on the pharmacological properties they possess, and the place where this enzymatic activity has been described for some species. In spite of their wide distribution in nature, most of these enzymes have been isolated from snakes, bees and scorpions venoms, as well as from pancreas tissues of mammals and membranes for example, those of mitochondria from hepatic tissues. These enzymes are widely spread in nature and their functional versatility and usefulness have increasingly conditioned their use in Specialized and Applied Biochemistry as well as biotechnological, pharmaceutical, and general industries such as food industry, where a highly purified enzyme is not always needed. It also reveals maximum activity when the substrate is organized in large molecular aggregations such as micelles. These enzymes yield a lyso derivative (lysophospholipid) and a free-fatty acid molecule, which is able to hydrolyze both natural and synthetic phospholipids regardless of the nature of the fatty acids in the molecule. ![]() Phospholipases A 2 (PLA 2) are esterolytic enzymes with high substrate and bond specificity for the hydrolysis of the sn-2 position of phosphoacylglycerols. Keywords: affinity, phospholipase A 2, Condylactis gigantea, sea anemone, protein purification. These components are produced with molecular weights between 1800, and at least one component possessing phospholipase A 2 activity. By means of the above-mentioned support, purification of three protein-based components can be carried out. The phospholipase A 2 activity was corroborated by using qualitative TLC and a fluorogenic substrate. PLA 2 purification from the sea anemone Condylactis gigantean is conducted through a chromatographic affinity support MANA-Sepharose CL 4B with covalently immobilized phosphatidylcholine egg. This research study aims to test a two-step purification procedure for phospholipases A 2 (PLA 2) to filter enzymes for further characterization. Marine coelenterate venom is composed of complex mixtures of several substances, mainly of proteins, for which phospholipase A 2 activity has been described. Palabras clave: cromatografía de afinidad, fosfolipasa A 2, anémona marina, Condylactis gigantea, purificación de proteínas. Dicho soporte cromatográfico permite que en un protocolo de purificación de solamente dos pasos se obtengan tres componentes proteicos de pesos moleculares entre 18000 y 14000, con al menos un componente que posee actividad fosfolipásica A 2. ![]() Se muestra la purificación de FLA 2 en un soporte cromatográfico de afinidad con fosfatidilcolina de huevo inmovilizada covalentemente para la purificación de fosfolipasas A 2, a los que se les comprobó cualitativamente la actividad fosfolipásica A 2 por cromatografía en placa (TLC) utilizando sustrato marcado con fluorescencia. El objetivo de la presente investigación fue purificar las fosfolipasas A 2 (FLA 2) procedentes de la anémona marina Condylactis gigantea a partir de un protocolo de dos etapas para su posterior caracterización. IFacultad de Ciencias Naturales, Universidad de Oriente, Santiago de Cuba, Cuba, de Estudio de Proteínas (CEP), Facultad de Biología, Universidad de La Habana, Ciudad Habana, Cuba, de Catálisis y Petroleoquímica, CSIC, Campus UAM, Madrid, España, de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, Porto Velho-RO, Brazil, veneno de los celenterados marinos constituyen mezclas complejas de varios componentes, muchos de naturaleza proteica, y para los cuales se ha descrito la actividad fosfolipásica A 2. Dolores Lázara Romero-Del-Sol I I, José Manuel Guisán-Seijas I I I, Joaquín Díaz-Brito II Dr.C. Protocolo de purificación en dos etapas de fosfolipasas A 2 a partir de la anémona marina Condylactis giganteaĪ Two-Step Purification Procedure of Phospholipases A 2 from the Sea Anemone Condylactis gigantea ![]() I noticed that it’s much easier to fill the shape of the blackberry with the small elements, starting with the bottom row. ![]() The rows are fitting closely to each other. Just imagine that the center of each drupe is sitting on the line you drew in the previous step. The reference lines are helpful for adding the rows of the drupes. With an HB pencil, I draw a rough shape of the blackberry then I add the parallel, slightly curved subsidiary lines.īerries like blackberries are composed of a cluster of small drupes around a central stem. It’s important to leave the highlights because a cherry is a smooth, glossy object. Then I add some graphite strokes to the stem and mark the drop shadow. I accentuate the hollow and the sides of the cherry with the contour hatching. I add a small hollow to the upper part of the cherry and refine the stem. This shape resembles a circle with a narrowed bottom part. I draw the shape of the cherry, using the HB pencil. This drawing already looks as if we’ve worked on it for a long time! 3. How to Draw a Cherry Step 1 Please be sure to leave a highlight on the upper part of the strawberry. I also emphasize the small hollows (where the seeds are located) and then add the drop shadow. I accentuate the sides of the strawberry, leaving the thin lines of the reflected light, and darken the leaves. It’s time to apply the hatching, using a 3B pencil. They arrange themselves in a staggered order. I soften the subsidiary lines with an eraser, so that they are barely visible, and draw the seeds. Our goal is to make an illusion of volume, so the lines should be slightly bent. I also add the subsidiary lines they will help us to allocate small seeds on the fruit’s body. I refine the shape of the strawberry and the contours of the leaves. Then I add the shapes of the leaves and the stem. With an HB pencil, I draw the rough shape of the strawberry. However, for the sake of this tutorial, let’s close our eyes to this fact. Technically, a strawberry is not a berry-it’s an aggregate fruit. If you don’t like using your own hand, you can get a blending stump or an ordinary cotton bud. Human skin has natural oils, so your drawing may get dirty. Please be sure that your hands are clean and dry before you make anything like this. To do that, I use my finger, gently rubbing the end of the hatching sample. Sometimes it’s great to smooth over the look of the graphite strokes, making them blurry. Try to vary the pressure and layer the graphite strokes you can get a dark surface which will seem almost black. I’m making lines with soft rubbing strokes, holding my 3B pencil at an angle. Let’s create a beautiful, velvety-looking texture. The lines made with the 3B pencil are darker and more intense, so this tool is the optimal choice for shading. The HB pencil leaves lighter marks on the paper, and we’ll use it mostly for creating the initial contours and designing the drawing. In my image, the HB sample is on the left, and the 3B is on the right. We have two types: an HB and a 3B.Ĭreate two samples of simple parallel hatching, just to observe how they look next to each other. Let’s compare the possibilities of our pencils. Step 2Īnother important technique for creating an illusion of three-dimensionality is contour hatching.Īs you can see in the image below, the lines are slightly bent, as if they are repeating the contours of an object. The first type of hatching is a group of straight lines that have the same direction and approximately equal length. We’ll observe different ways of applying strokes and provide a starting point for developing your skill set. This part of the tutorial is devoted to the tips and tricks of using graphite pencils. In order to complete this tutorial, you'll need the following equipment:ġ. How to Draw With a Graphite Pencil Step 1 ![]() Nothing benefits an artist more than thoughtful observation and daily practice! What You Will Need To understand and repeat the steps, you just need to become familiar with the hatching techniques and observe my illustrations closely. You don't need anything fancy to complete this tutorial just two pencils, an eraser, several sheets of drawing paper, and, of course, a desire for experimenting with your art supplies and creativity!Ī background in drawing is desirable but not obligatory. We’ll also learn to apply different types of hatching to achieve impressive results. In this tutorial, I’ll show you an easy and quick way to sketch with graphite pencils. ![]() Basic but hearty Creole and international meals are served by the helpful crew in the dining area on the main deck.Many experienced divers consider liveaboards and dive safaris as the only way to truly enjoy a diving vacation, especially if heading to remote destinations such as the Similan Islands in Thailand or Osprey Reef in Australia’s Coral Sea. This feature also allows for a safe and comfortable transfer from the tender boats to the main vessel. The stern of the vessel has a set of stairs leading down to a large swimming and fishing plat-form. The lower deck’s primary function is to store the tender boats and fishing tackle. This area is perfect for sundowners and socializing. On the mid-deck there is a spacious partially-covered seating area with magnificent views that is used often by guests. Both the cabins and indoor dining areas are fully air-conditioned, allowing guests a chance to relax in a comfortable environment after a long day’s fishing on the flats. There is also a comfortable salon and dining area. Each cabin has an en-suite shower and flush toilet. It is a four-level vessel and can accommodate 12 anglers in seven snug guest cabins situated either on the middle, main or lower deck. The ship is a bit rustic, but it is large, spacious and extremely stable, making it a perfect base from which to explore the remote outer atolls of the Seychelles. The Mayas Dugong is an ex-research vessel that has been upgraded and modified into a mothership specializing in long-range fly fishing expeditions. Guests will often spend long periods away from the boat, making this a physically rigorous trip. All anglers will spend the majority of their time wading. During a specific shift in tides they might make the call to look for milkfish, and during slack tide they might decide to wind-drift through coral heads blind casting for a wide range of hard fighting fish including grouper and bluefin trevally. When the conditions offer minimal tidal movement they might choose to spend the day walking the most productive flats for bonefish, permit, triggerfish and bumphead parrotfish. When the tides are big, they might elect to spend most of the day targeting giant trevally. Due to their intimate knowledge of tides and fish behavior, each day the guides will decide which type of fishing to focus on. ![]() The mothership carries four large sea-worthy skiffs, each captained by an experienced South African guide, that fish three per boat. The Providence fishing program is based on 12 anglers. Add to that a chance at Napoleon wrasse, Bohar snapper, barracuda, sailfish and multiple species of tuna and it won’t take long to recognize this as one of the greatest multi-species destinations our sport has to offer. Anglers can expect to target enormous giant trevally, bonefish, triggerfish, Indo-Pacific permit, milkfish, and bumphead parrot-fish. Providence offers fly fishermen the opportunity to wade and sight fish a possible 29 miles of pristine sand and turtle grass flats, an enormous lagoon system as well as the numerous channels which link these structures to the Indian Ocean. The sheer size and amount of habitat, both on the flats and offshore, create an endless array of angling options. For the consummate saltwater fly fishing professionals and explorers at Fl圜astaway, this complex is truly their providence the largest, most diverse and exciting saltwater wilderness on earth. Located 440 miles south of the capital city of Mahé, Providence is a large uninhabited complex consisting of two islands (North Island and Cerf Island), and a vast array of flats in-between that has received less than 1,000 total visitors in its recorded history. ![]() Providence is a specific atoll within the Seychelles archipelago located in the Indian ocean east of Tanzania and north of Madagascar. For those that are serious about finding the most pristine and rewarding flats fishing in the world today, all points lead to here. Providence, after all, is one’s fate, destiny or divine predestination. For the most committed and adventuresome saltwater anglers, it is appropriate and even fortunate that the wildest and most productive ecosystem in the Seychelles, and likely the world, is named Providence. Toutousai is the creator of two of the four most powerful swords in the story: Inuyasha's Tessaiga, accurately reputed to be capable of killing a hundred youkai with a single swing, and Sesshoumaru's Tenseiga, just as accurately reputed to be capable of saving a hundred lives with a single swing.Winry, however, eventually leaves to study in Rush Valley, a booming haven for automail engineers. ![]()
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Physician Joseph Price, who figures prominently in our article on the Garretson Hospital, wrote an article in 1889 documenting 500 deliveries at the Preston Retreat without a single case of septicemia or death. Another reason may be favorable patient selection, since patients required "testimonials" before admission, and those without presumably went to the Blockley Almshouse in West Philadelphia, which opened its massive new facility in 1835 (the future Philadelphia General Hospital, and then the site of CHOP). The Preston Retreat had regulations that prevented the training of medical students there, which may have been one reason for the lower infection rate. The notion that doctors might be spreading disease did not sit well with doctors, and attention to the advice of Holmes and Semmelweis was not widespread until the 1860s, when Louis Pasteur showed a biologic mechanism for transmission of infectious disease via germs, and Joseph Lister showed a way to make instruments and procedures more antiseptic via using carbolic acid sprays. At this time germ theory was not generally known. He recommended, in addition to Holmes' advice, that doctors wash their hands in a bleach solution before assisting in deliveries. ![]() In 1847, Ignaz Semmelweis, a Hungarian obstetrician, noted that women who delivered at home or in a hospital with midwives had a much lower risk of infection than mothers delivering in a hospital with physicians and medical students. In it he suggested that physicians themselves were transferring the infection between patients, and recommended avoiding assistance at childbirth immediately after performing an autopsy. In 1843 Oliver Wendell Holmes Sr., the father of the future US Supreme Court justice, published his monograph The Contagiousness of Puerperal Fever. ![]() For example, in 1854 an outbreak of childbed fever closed the maternity hospital at Pennsylvania Hospital on Spruce Street. Throughout the world, maternal mortality was dreadful, in large part due to infection, until the latter half of the 19th century. The Retreat had a deserved reputation for a low infection rate and mortality. ![]() William Goodell, it admitted pregnant women 16 days before their due date, offering clean clothes, good meals, and two baths per week. The wealthy had the privilege of delivering in their own homes. Andrews Catholic Lithuanian Church today) the Gynecean Hospital for gynecology care at 18th and Vine (now the Holy Family Center annex to the Cathedral) the Indigent Widows and Single Women's Society and adjacent orphanage at 19th and Cherry (now the Logan Hotel) and the Magdalen Society of Philadelphia, an "institution for the shelter and reformation of fallen women," at the northeast corner of 21st and Race (on land now part of The Franklin Institute). Not to be outdone in spirit if not in dollar amount, in 1836 Preston left $250,000 (today $6 million) to the State for the construction and maintenance of a lying-in hospital for indigent married women of good character. Per his will: "The persons to be admitted shall be married women of good character, and in indigent circumstances, who are near the time of their confinement and at the time of application shall be resident in the city or county of Philadelphia or county of Delaware, and shall produce satisfactory testimonials of character." The location chosen may seem a bit out of the way, but in this area in the 1800s there were several centers of women's care: the City Hospital for general care at 19th and Wallace (site of St. Apparently philanthropy was in vogue in the early 1830s, with Stephen Girard in 1831 leaving the City of Philadelphia $6 million (inflation adjusted $140 million today) to fund a private K-12 boarding school for poor white male orphans. This lying-in hospital was funded by the estate of Jonas Preston, a Philadelphia physician, politician, and philanthropist. These columns are the remnants of the Preston Retreat, a maternity hospital which began construction in 1837, functioned as a foster home from 1840 to 1865, and finally opened for maternity services in 1866. ![]() In a letter to his charitable foundation around this time, Wexner claimed Epstein had “misappropriated vast sums of money from me and my family.” Epstein had reportedly transferred back nearly $47 million to a Wexner-controlled charity fund in 2008.īut Wexner’s belated attempts to explain himself only raised more questions. “Being taken advantage of by someone who was so sick, so cunning, so depraved is something that I’m embarrassed that I was even close to,” Wexner said during a speech in September 2019. In Wexner’s telling, he was another Epstein victim preyed upon by a devious mastermind. ![]() It was only after Epstein died that Wexner described their relationship-and even then in opaque terms. They condemned Epstein’s actions, the spokesman said. In 2019, a Wexner spokesman told The Washington Post that the Wexners had severed all ties with Epstein in 2007. When Epstein pleaded guilty to two counts, including soliciting a minor for prostitution, in Palm Beach in June 2008 and registered as a Level 3 sex offender in New York, Wexner refused to discuss him. Leslie Wexner with his wife Abigail Wexner and son Harry Wexner during the Royal Shakespeare Company performance of "Romeo and Juliet" at the Park Avenue Armory in New York, July 12, 2011. In other words, Epstein became Epstein during his long association with Wexner. The building was formerly owned by Wexner.) Prosecutors say that Epstein built his vast sex-trafficking ring throughout the ’90s and early aughts. (Epstein’s brother’s real estate company also had majority ownership of a Manhattan condo building on East 66th Street where Epstein allegedly housed girls. His estate included a 51,000-square-foot Manhattan town house (bought from Wexner) a private jet (formerly owned by The Limited) and a helicopter a Caribbean island a Paris apartment a Palm Beach mansion and a 10,000-acre New Mexico ranch. Epstein, a former high school math teacher from Coney Island, Brooklyn, was worth a reported $559 million. Wexner paid Epstein’s predecessor about $600,000 a year in today’s dollars. “When I asked Jeffrey who else he worked with, he’d say, ‘I can’t talk about it,’ ” an Epstein friend recalled. But until recently, Epstein’s only publicly named client was Wexner. “There’s no question that Epstein could not have done what he did without the support of people that provided him money,” said attorney David Boies, whose firm represents numerous Epstein accusers, including Virginia Roberts Giuffre.Įpstein burnished his own myth, telling people, preposterously, that he only accepted clients with assets of $1 billion or more. In a search for answers, I spent the past six months investigating the Epstein mystery that could unlock others: How did he get his money? For it was Epstein’s half-billion-dollar fortune that enabled him to sexually abuse and traffic hundreds of girls on multiple continents. Was Epstein an intelligence agent? A debased financial genius? A sociopathic con man who fleeced billionaires and politicians with sexual blackmail? Two years after guards discovered Epstein unconscious in his Manhattan prison cell, the pedophile’s life and mysterious death remain a subject of fevered speculation and conspiracy theorizing. He applied for jobs using computers at the library and showered at state parks. I was living out of my car for a while,” Levin said. His wife filed for divorce, Levin said, and their three children stopped speaking to him. Friends told him Epstein was spreading rumors around Columbus and on Wall Street that Wexner had fired him for misappropriating funds. “Les sent me to Richmond, Virginia, to look at architecture he wanted to copy,” Levin said. Starting in the mid-1980s, Levin purchased thousands of acres of farmland in New Albany (population 414) on the outskirts of Columbus, where Wexner planned to build his very own town modeled on an 18th-century Georgian village. Wexner entrusted Levin with increasingly ambitious projects. “The kitchen was so clean, you could eat off the floor,” Levin remembered. Once, the manager of the Regency Hotel arranged a private tour of Le Bernardin. “In New York, I had bankers constantly taking me out to dinner,” Levin told me. By 1986, Wexner ranked sixth on the Forbes 400 list of richest Americans, with a net worth estimated at $1.4 billion. ![]() He wasn’t making Masters of the Universe money-Wexner paid a salary of $250,000 a year-but it was enough for Levin to move his family into a 6,000-square-foot house across the street from Wexner in Bexley, Columbus’s most exclusive suburb. Levin landed the job after six months of grueling interviews. ![]() This is a full offline installer standalone setup for Windows Operating System. VideoHive – Wedding Parallax Slideshow Free DownloadĬlick on the link below to start the VideoHive – Wedding Parallax Slideshow Free Download. 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